electrophysiology data analysis  (GraphPad Software Inc)

Journal: Advanced Science

Article Title: A Microclip Peripheral Nerve Interface (μcPNI) for Bioelectronic Interfacing with Small Nerves

doi: 10.1002/advs.202102945

Figure Lengend Snippet: Chronic in vivo recording of nerve activity. a) Representative example of chronic TSN recording aligned to the song. (Top) Spectrogram of the bird's song; color indicates power intensity at each time‐frequency bin. (Bottom) Electrophysiology activity recorded from the TSN simultaneously with the song motif shown at top; timescale aligned to Figure at the bottom. Color here and in all other subpanels indicates the recording channel. b) Mean song‐aligned TSN activity envelope over n = 10 consecutive motifs for each channel. Same animal as shown in (a). c) Matrix of pair‐wise correlations between mean song‐aligned activity envelopes from each channel (n = 50 each channel; 300 total) for the representative animal shown in (a). Row and column relations to channel indicated by colored lines at left and bottom. d) Mean pair‐wise correlation between nerve activity envelopes recorded within (left) and across (right) μcPNI electrode in n = 3 birds. In each, data points show mean across electrodes within a bird. Bars show mean across all birds; error bars indicate std. ** P < 0.01 two‐tailed paired t‐test, P = 0.008. e) Metrics demonstrating stable performance of each μcPNI channel over time. (Left) Mean daily trial‐by‐trial Pearson's correlation to the average activity pattern on the 1st day of recording. (Center) Mean daily peak‐to‐peak voltage. (Right) Mean daily event rate. f) Same as in (e), but showing mean statistics across all electrode channels in n = 3 birds (two‐tailed paired t‐test; correlation: P > 0.17; V pp : P > 0.68; event rate: P > 0.37).

Article Snippet: All electrophysiology data analysis was performed off‐line using MATLAB.

Techniques: In Vivo, Activity Assay, Two Tailed Test